› 6gf8

X-ray diffraction
3.1Å resolution

Molecular basis of egg coat filament cross-linking: structure of the glycosylated ZP1 ZP-N1 domain homodimer

Released:
DOI: 10.2210/pdb6gf8/pdb
PDB entry 6gf8 coloured by chain, front view.
PDB entry 6gf8 coloured by chain, side view.
PDB entry 6gf8 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.
Nishimura K, Dioguardi E, Nishio S, Villa A, Han L, Matsuda T, Jovine L
Nat Commun 10 3086 (2019)
PMID: 31300655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Zona pellucida sperm-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.49 KDa
Source organism: Gallus gallus
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A140JXP0 (Residues: 24-139, 141-149; Coverage: 13%)
Gene name: ZP1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 90.774Å b: 125.734Å c: 105.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.235
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Human Zona Pellucida Glycoproteins: Binding Characteristics With Human Spermatozoa and Induction of Acrosome Reaction.
Gupta SK. (2021)
8 more

1 mention without citation

Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.
Nishimura et al. (2019)