› 6gcu

X-ray diffraction
6Å resolution

MET receptor in complex with InlB internalin domain and DARPin A3A

Released:
DOI: 10.2210/pdb6gcu/pdb
PDB entry 6gcu coloured by chain, front view.
PDB entry 6gcu coloured by chain, side view.
PDB entry 6gcu coloured by chain, top view.
Source organisms:
Primary publication:
Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking.
Andres F, Iamele L, Meyer T, Stüber JC, Kast F, Gherardi E, Niemann HH, Plückthun A
J Mol Biol 431 2020-2039 (2019)
PMID: 30930049

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140194 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hepatocyte growth factor receptor Chains: A, D
Molecule details ›
Chains: A, D
Length: 727 amino acids
Theoretical weight: 81.95 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08581 (Residues: 25-741; Coverage: 53%)
Gene name: MET
Sequence domains:
Internalin B Chains: B, E
Molecule details ›
Chains: B, E
Length: 289 amino acids
Theoretical weight: 32.24 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
DARPin A3A Chains: C, F
Molecule details ›
Chains: C, F
Length: 173 amino acids
Theoretical weight: 18.51 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P31
Unit cell:
a: 144.874Å b: 144.874Å c: 128.896Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.262 0.271
Expression systems:
  • Cricetulus griseus
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21

Quick links

Citations

2 review citations

Gastric Cancer: Advances in Carcinogenesis Research and New Therapeutic Strategies.
Seeneevassen et al. (2021)
1 more

3 mentions without citation

State of the structure address on MET receptor activation by HGF.
Linossi et al. (2021)
2 more