› 6gbv

X-ray diffraction
1.63Å resolution

A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49T mutant

Released:
DOI: 10.2210/pdb6gbv/pdb
PDB entry 6gbv coloured by chain, front view.
PDB entry 6gbv coloured by chain, side view.
PDB entry 6gbv coloured by chain, top view.
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Primary publication:
Mechanistic Basis of the Fast Dark Recovery of the Short LOV Protein DsLOV from Dinoroseobacter shibae.
Fettweiss T, Röllen K, Granzin J, Reiners O, Endres S, Drepper T, Willbold D, Jaeger KE, Batra-Safferling R, Krauss U
Biochemistry 57 4833-4847 (2018)
PMID: 29989797

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative blue-light photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.83 KDa
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A8LP63 (Residues: 2-139; Coverage: 99%)
Gene name: Dshi_2006
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 90.076Å b: 31.086Å c: 49.411Å
α: 90° β: 112.88° γ: 90°
R-values:
R R work R free
0.15 0.148 0.198
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

4 citation in other articles

A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies.
Nazarenko et al. (2019)
3 more