6g9v

X-ray diffraction
1.75Å resolution

Crystal structure of Aspergillus fumigatus UDP-N-acetylglucosamine pyrophosphorylase(AfUAP1) in complex with UDPGlcNAc, pyrophosphate and Mg2+

Released:

Function and Biology Details

Reaction catalysed:
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine pyrophosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 509 amino acids
Theoretical weight: 56.79 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WAR0 (Residues: 1-509; Coverage: 100%)
Gene name: AFUA_7G02180
Sequence domains: UTP--glucose-1-phosphate uridylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 55.195Å b: 139.839Å c: 144.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.24
Expression system: Escherichia coli