X-ray diffraction
1.45Å resolution

Crystal structure of the light chain of botulinum neurotoxin X (residues 2-442)

Source organism: Clostridium botulinum
Entry authors: Wu Y, Kammerer R

Function and Biology Details

Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Botulinum neurotoxin X light chain Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 52.17 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0DPK1 (Residues: 2-442; Coverage: 34%)
Gene name: CBB2_0680
Sequence domains: Clostridial neurotoxin zinc protease

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 60.32Å b: 87.26Å c: 93.85Å
α: 90° β: 90° γ: 90°
R R work R free
0.146 0.144 0.182
Expression system: Escherichia coli BL21(DE3)