› 6g4j

X-ray diffraction
1.6Å resolution

Structure of the protein kinase YabT from Bacillus subtilis in complex with an alphaREP crystallization helper

Released:
DOI: 10.2210/pdb6g4j/pdb
PDB entry 6g4j coloured by chain, front view.
PDB entry 6g4j coloured by chain, side view.
PDB entry 6g4j coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analysis of the Hanks-Type Protein Kinase YabT From Bacillus subtilis Provides New Insights in its DNA-Dependent Activation.
Shi L, Cavagnino A, Rabefiraisana JL, Lazar N, Li de la Sierra-Gallay I, Ochsenbein F, Valerio-Lepiniec M, Urvoas A, Minard P, Mijakovic I, Nessler S
Front Microbiol 9 3014 (2018)
PMID: 30671027

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153431 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Probable serine/threonine-protein kinase YabT Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.02 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37562 (Residues: 1-315; Coverage: 93%)
Gene names: BSU00660, yabT
Sequence domains: Protein kinase domain
alphaREP bE8 Chain: B
Molecule details ›
Chain: B
Length: 148 amino acids
Theoretical weight: 15.86 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 67.69Å b: 122.87Å c: 50.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.217
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Structural and Functional Insights into the Biofilm-Associated BceF Tyrosine Kinase Domain from Burkholderia cepacia.
Mayer et al. (2021)

2 mentions without citation

Structural Analysis of the Hanks-Type Protein Kinase YabT From Bacillus subtilis Provides New Insights in its DNA-Dependent Activation.
Shi et al. (2018)
1 more