6g3u

X-ray diffraction
2.71Å resolution

Structure of Pseudomonas aeruginosa Isocitrate Dehydrogenase, IDH

Released:

Function and Biology Details

Reaction catalysed:
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 737 amino acids
Theoretical weight: 81.25 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q9I0L4 (Residues: 5-741; Coverage: 100%)
Gene names: PA2624, idh
Sequence domains: Monomeric isocitrate dehydrogenase

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 126.46Å b: 149.02Å c: 201.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.267
Expression system: Escherichia coli DH5[alpha]