6g32

X-ray diffraction
3.28Å resolution

Crystal structure of human geranylgeranyl diphosphate synthase mutant D188Y

Released:

Function and Biology Details

Reactions catalysed:
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranylgeranyl pyrophosphate synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 307 amino acids
Theoretical weight: 35.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95749 (Residues: 1-300; Coverage: 100%)
Gene name: GGPS1
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 148.54Å b: 148.54Å c: 268.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.258
Expression system: Escherichia coli