› 6g1b

X-ray diffraction
2.28Å resolution

Corynebacterium glutamicum OxyR, oxidized form

Released:
DOI: 10.2210/pdb6g1b/pdb
PDB 6g1b coloured by chain and viewed from the front.
PDB 6g1b coloured by chain and viewed from the side.
PDB 6g1b coloured by chain and viewed from the top.
Source organism: Corynebacterium glutamicum
Primary publication:
Structural snapshots of OxyR reveal the peroxidatic mechanism of H2O2 sensing.
Pedre B, Young D, Charlier D, Mourenza Á, Rosado LA, Marcos-Pascual L, Wahni K, Martens E, G de la Rubia A, Belousov VV, Mateos LM, Messens J
OpenAccess logo Proc Natl Acad Sci U S A 115 E11623-E11632 (2018)
PMID: 30463959

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HTH lysR-type domain-containing protein Chain: J
Molecule details ›
Chain: J
Length: 327 amino acids
Theoretical weight: 35.09 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NP91 (Residues: 1-324; Coverage: 99%)
Gene name: Cgl1925
Sequence domains:
HTH lysR-type domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 326 amino acids
Theoretical weight: 35 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NP91 (Residues: 1-323; Coverage: 99%)
Gene name: Cgl1925
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand NI 1 x NI
Ligand FMT 3 x FMT
Ligand 1PS 1 x 1PS
Ligand NA 1 x NA
Ligand PEO 3 x PEO
1 modified residue:
Ligand SME 1 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 161.307Å b: 46.186Å c: 116.692Å
α: 90° β: 121.92° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Oxidative Stress-Generating Antimicrobials, a Novel Strategy to Overcome Antibacterial Resistance.
Mourenza et al. (2020)
1 more

1 mention without citation

Structural snapshots of OxyR reveal the peroxidatic mechanism of H2O2 sensing.
Pedre et al. (2018)