› 6g0p

X-ray diffraction
1.3Å resolution

Crystal Structure of the first bromodomain of human BRD4 in complex with an acetylated E2F1 peptide (K117ac/K120ac)

Released:
DOI: 10.2210/pdb6g0p/pdb
PDB 6g0p coloured by chain and viewed from the front.
PDB 6g0p coloured by chain and viewed from the side.
PDB 6g0p coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
Mol. Cell (2018)
PMID: 30554943

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 42-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Transcription factor E2F1 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q01094 (Residues: 114-129; Coverage: 4%)
Gene names: E2F1, RBBP3

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 44.137Å b: 53.358Å c: 58.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.125 0.147
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Proximity Labeling Techniques to Study Chromatin.
Ummethum et al. (2020)
2 more