› 6g0n

X-ray diffraction
1.8Å resolution

Crystal Structure of a GH8 catalytic mutant xylohexaose complex xylanase from Teredinibacter turnerae

Released:
DOI: 10.2210/pdb6g0n/pdb
PDB entry 6g0n coloured by chain, front view.
PDB entry 6g0n coloured by chain, side view.
PDB entry 6g0n coloured by chain, top view.
Source organism: Teredinibacter turnerae T7901
Primary publication:
Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae.
Fowler CA, Hemsworth GR, Cuskin F, Hart S, Turkenburg J, Gilbert HJ, Walton PH, Davies GJ
Acta Crystallogr D Struct Biol 74 946-955 (2018)
PMID: 30289404

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
cellulase Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.11 KDa
Source organism: Teredinibacter turnerae T7901
Expression system: Escherichia coli
UniProt:
  • Canonical: C5BJ89 (Residues: 41-436; Coverage: 97%)
Gene name: TERTU_4506
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand XYP 1 x XYP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 60.973Å b: 79.694Å c: 88.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.199
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries.
Ellilä et al. (2019)
2 more

1 mention without citation

Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae.
Fowler et al. (2018)