6fzs

X-ray diffraction
2.31Å resolution

Crystal structure of Smad5-MH1 bound to the GGCGC site.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99717 (Residues: 9-138; Coverage: 28%)
Gene names: MADH5, SMAD5
Sequence domains: MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*GP*CP*GP*CP*GP*CP*CP*TP*GP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 67.764Å b: 73.374Å c: 89.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.241
Expression systems:
  • Escherichia coli
  • Not provided