› 6fyb

X-ray diffraction
1.88Å resolution

The crystal structure of EncM L144M mutant

Released:
DOI: 10.2210/pdb6fyb/pdb
PDB 6fyb coloured by chain and viewed from the front.
PDB 6fyb coloured by chain and viewed from the side.
PDB 6fyb coloured by chain and viewed from the top.
Source organism: Streptomyces maritimus
Primary publication:
Enzymatic control of dioxygen binding and functionalization of the flavin cofactor.
Saleem-Batcha R, Stull F, Sanders JN, Moore BS, Palfey BA, Houk KN, Teufel R
Proc Natl Acad Sci U S A 115 4909-4914 (2018)
PMID: 29686059

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 464 amino acids
Theoretical weight: 50.09 KDa
Source organism: Streptomyces maritimus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KHK2 (Residues: 1-464; Coverage: 100%)
Gene name: encM
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C222
Unit cell:
a: 161.993Å b: 174.953Å c: 130.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Enzymatic Cascade Reactions in Biosynthesis.
Walsh et al. (2019)
1 more