6fud

X-ray diffraction
1.3Å resolution

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikA with the HMA domain of Pikm-1 from rice (Oryza sativa)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Disease resistance protein Pikm1-TS Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 8.55 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: B5UBC1 (Residues: 186-264; Coverage: 7%)
Gene names: PI-KM1, PIKM1-TS
Structure domains: Alpha-Beta Plaits
AVR-Pik Chain: B
Molecule details ›
Chain: B
Length: 93 amino acids
Theoretical weight: 10.84 KDa
Source organism: Pyricularia oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: C4B8B9 (Residues: 22-113; Coverage: 100%)
Gene names: AVR-Pik, AVR-Pikm

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 30.07Å b: 54.44Å c: 46.64Å
α: 90° β: 104.43° γ: 90°
R-values:
R R work R free
0.127 0.125 0.162
Expression system: Escherichia coli