› 6fsk

X-ray diffraction
1.56Å resolution

F194Y mutant of the Dye-decolorizing peroxidase (DYP) from Pleurotus ostreatus

Released:
DOI: 10.2210/pdb6fsk/pdb
PDB entry 6fsk coloured by chain, front view.
PDB entry 6fsk coloured by chain, side view.
PDB entry 6fsk coloured by chain, top view.
Source organism: Pleurotus ostreatus PC15
Primary publication:
Description of a non-canonical Mn(II)-oxidation site in peroxidases
Fernandez-Fueyo E, Davo-Siguero I, Almendral D, Linde D, Baratto MC, Pogni R, Romero A, Guallar V, Martinez AT
ACS Catalysis (2018)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DyP-type peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 53.14 KDa
Source organism: Pleurotus ostreatus PC15
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: A0A067N4E7 (Residues: 6-489; Coverage: 96%)
Gene names: DyP4, PLEOSDRAFT_1069077
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand MES 1 x MES
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 62.717Å b: 117.575Å c: 140.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.199
Expression system: Escherichia coli #1/H766

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