6fqm

X-ray diffraction
3.06Å resolution

3.06A COMPLEX OF S.AUREUS GYRASE with imidazopyrazinone T1 AND DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
DNA gyrase subunit B Chains: B, b, d
Molecule details ›
Chains: B, b, d
Length: 201 amino acids
Theoretical weight: 22.47 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: P66937 (Residues: 410-644; Coverage: 31%)
Gene names: SA0005, gyrB
Sequence domains: Toprim domain
DNA gyrase subunit A Chains: A, C, a, c
Molecule details ›
Chains: A, C, a, c
Length: 490 amino acids
Theoretical weight: 55.62 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99XG5 (Residues: 2-491; Coverage: 55%)
Gene names: SA0006, gyrA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA gyrase subunit B Chain: D
Molecule details ›
Chain: D
Length: 202 amino acids
Theoretical weight: 22.61 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: P66937 (Residues: 409-644; Coverage: 31%)
Gene names: SA0005, gyrB
Sequence domains: Toprim domain
DNA (5'-D(*GP*AP*GP*AP*GP*TP*AP*T*GP*GP*CP*CP*AP*TP*AP*CP*TP*CP*T)-3') Chains: E, F, e, f
Molecule details ›
Chains: E, F, e, f
Length: 20 nucleotides
Theoretical weight: 6.15 KDa

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 88.34Å b: 171.71Å c: 124.87Å
α: 90° β: 102.86° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli