6fq8

Electron Microscopy
4.8Å resolution

Class 3 : translocated nucleosome

Released:
Source organism: Xenopus laevis
Primary publication:
Structural rearrangements of the histone octamer translocate DNA.
OpenAccess logo Nat Commun 9 1330 (2018)
PMID: 29626188
Related structures: EMD-4299

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H3.3C Chains: A, E
Molecule details ›
Chains: A, E
Length: 98 amino acids
Theoretical weight: 11.56 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02302 (Residues: 38-135; Coverage: 72%)
Gene names: h3-5, h3f3c
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 86 amino acids
Theoretical weight: 9.86 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 18-103; Coverage: 84%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 110 amino acids
Theoretical weight: 12.08 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 10-119; Coverage: 85%)
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 95 amino acids
Theoretical weight: 10.64 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 31-125; Coverage: 75%)
DNA (147-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.6 KDa
DNA (147-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.15 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-4299
Expression system: Escherichia coli