6fq3

X-ray diffraction
1.9Å resolution

Crystal structure of Danio rerio Lin41 filamin-NHL domains in complex with lin-29A 5'UTR 13mer RNA

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase TRIM71 Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 44.62 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: E7FAM5 (Residues: 435-824; Coverage: 47%)
Gene names: lin41, trim71
Sequence domains:
RNA (5'-R(*GP*GP*AP*GP*UP*CP*CP*AP*AP*CP*UP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.12 KDa
Source organism: Caenorhabditis elegans
Expression system: synthetic construct

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 101.85Å b: 101.85Å c: 109.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.18
Expression systems:
  • Spodoptera frugiperda
  • synthetic construct