› 6fpa

X-ray diffraction
1.58Å resolution

Crystal structure of anti-mTFP1 DARPin 1238_G01 in space group P212121

Released:
DOI: 10.2210/pdb6fpa/pdb
PDB entry 6fpa coloured by chain, front view.
PDB entry 6fpa coloured by chain, side view.
PDB entry 6fpa coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations.
Vigano MA, Bieli D, Schaefer JV, Jakob RP, Matsuda S, Maier T, Plückthun A, Affolter M
Biol Open 7 (2018)
PMID: 30237292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DARPin 1238_G01 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.36 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 37.99Å b: 89.156Å c: 103.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Reflections on the use of protein binders to study protein function in developmental biology.
Aguilar et al. (2019)
3 other articles

2 mentions without citation

DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations.
Vigano et al. (2018)
1 more