› 6fo1

Electron Microscopy
3.57Å resolution

Human R2TP subcomplex containing 1 RUVBL1-RUVBL2 hexamer bound to 1 RBD domain from RPAP3.

Released:
DOI: 10.2210/pdb6fo1/pdb
PDB entry 6fo1 coloured by chain, front view.
PDB entry 6fo1 coloured by chain, side view.
PDB entry 6fo1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex.
Martino F, Pal M, Muñoz-Hernández H, Rodríguez CF, Núñez-Ramírez R, Gil-Carton D, Degliesposti G, Skehel JM, Roe SM, Prodromou C, Pearl LH, Llorca O
Nat Commun 9 1501 (2018)
PMID: 29662061
Related structures: EMD-4287

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-190647 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 456 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
RuvB-like 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 463 amino acids
Theoretical weight: 51.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:
RNA polymerase II-associated protein 3 Chain: G
Molecule details ›
Chain: G
Length: 665 amino acids
Theoretical weight: 75.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H6T3 (Residues: 1-665; Coverage: 100%)
Gene name: RPAP3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.57Å
Relevant EMDB volumes: EMD-4287
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Methods review: Mass spectrometry analysis of RNAPII complexes.
Burriss et al. (2019)
5 more

5 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
4 more