PDBe 6fnn

X-ray diffraction
1.85Å resolution

Caldiarchaeum Subterraneum Ubiquitin:Rpn11-homolog complex

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MPN domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.06 KDa
Source organism: Candidatus Caldiarchaeum subterraneum
Expression system: Escherichia coli
UniProt:
  • Canonical: E6N8B9 (Residues: 2-148; Coverage: 73%)
Gene names: CSUB_C1473, HGMM_F04B03C04, HGMM_F21D07C20, HGMM_F30C12C32
Ubiquitin-like domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 104 amino acids
Theoretical weight: 11.37 KDa
Source organism: Candidatus Caldiarchaeum subterraneum
Expression system: Escherichia coli
UniProt:
  • Canonical: E6N8B8 (Residues: 1-78; Coverage: 90%)
Gene names: CSUB_C1474, HGMM_F04B03C03, HGMM_F21D07C21, HGMM_F30C12C33
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 84.674Å b: 94.571Å c: 170.855Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.201
Expression system: Escherichia coli