X-ray diffraction
3Å resolution

Function and Biology Details

Reaction catalysed:
Release of signal peptides from bacterial membrane prolipoproteins including murein prolipoprotein. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral sidechains.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lipoprotein signal peptidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 188 amino acids
Theoretical weight: 21.29 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9HVM5 (Residues: 2-169; Coverage: 99%)
Gene names: PA4559, ls, lspA
Sequence domains: Signal peptidase (SPase) II
Globomycin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 5 amino acids
Theoretical weight: 674 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

4 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 112.75Å b: 110.13Å c: 85.99Å
α: 90° β: 97.08° γ: 90°
R R work R free
0.23 0.228 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided