X-ray diffraction
1.58Å resolution

Targeting myeloid differentiation using potent human dihydroorotate dehydrogenase (hDHODH) inhibitors based on 2-hydroxypyrazolo[1,5-a]pyridine scaffold

Source organism: Homo sapiens
Entry authors: Goyal P, Jarva M, Andersson M, Lolli ML, Friemann R

Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 42.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q02127 (Residues: 1-395; Coverage: 100%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments

Cofactor: Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 90.441Å b: 90.441Å c: 122.446Å
α: 90° β: 90° γ: 120°
R R work R free
0.138 0.137 0.16
Expression system: Escherichia coli BL21(DE3)