› 6fll

X-ray diffraction
1.79Å resolution

SPECTROSCOPIC AND STRUCTURAL STUDY OF QW, A EGFP MUTANT SHOWING PHOTOSWITCHING PROPERTIES

Released:
DOI: 10.2210/pdb6fll/pdb
PDB entry 6fll coloured by chain, front view.
PDB entry 6fll coloured by chain, side view.
PDB entry 6fll coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Role of Gln222 in Photoswitching of Aequorea Fluorescent Proteins: A Twisting and H-Bonding Affair?
Storti B, Margheritis E, Abbandonato G, Domenichini G, Dreier J, Testa I, Garau G, Nifosì R, Bizzarri R
ACS Chem Biol 13 2082-2093 (2018)
PMID: 29878744

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.55 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYS 1 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 51.45Å b: 62.71Å c: 66.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.232
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data.
Moeyaert et al. (2020)
5 more