› 6fl1

X-ray diffraction
1.6Å resolution

Crystal structure of the complex between the Lactococcus lactis FPG mutant T221P and a Fapy-dG containing DNA

Released:
DOI: 10.2210/pdb6fl1/pdb
PDB entry 6fl1 coloured by chain, front view.
PDB entry 6fl1 coloured by chain, side view.
PDB entry 6fl1 coloured by chain, top view.
Source organisms:
Entry authors: Coste F, Castaing B, Ober M, Carell T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119908 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.11 KDa
Source organism: Lactococcus cremoris
Expression system: Escherichia coli
Structure domains:
DNA (5'-D(*CP*TP*CP*TP*TP*TP(FOX)P*TP*TP*TP*CP*TP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.36 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand FOX 1 x FOX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41212
Unit cell:
a: 91.369Å b: 91.369Å c: 141.673Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.178
Expression systems:
  • Escherichia coli
  • Not provided

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