Structure analysis

Structure of 3' phosphatase NExo (D146N) from Neisseria bound to DNA substrate in presence of magnesium ion

X-ray diffraction
2.02Å resolution
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 12500 Å2
Buried surface area: 1900 Å2
Dissociation area: 750 Å2
Dissociation energy (ΔGdiss): 6 kcal/mol
Dissociation entropy (TΔSdiss): 10 kcal/mol
Interface energy (ΔGint): -28 kcal/mol
Symmetry number: 1

Macromolecules

Chain: A
Length: 258 amino acids
Theoretical weight: 29.32 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9K100 (Residues: 1-256; Coverage: 100%)
Gene names: NMB0399, xthA
Pfam: Endonuclease/Exonuclease/phosphatase family
InterPro:
CATH: Endonuclease/exonuclease/phosphatase

Search similar proteins

Name: DNA (5'-D(P*CP*TP*AP*GP*CP*GP*AP*AP*GP*CP*TP*AP*GP*A)-3')
Representative chains: B
Source organism: other sequences [28384]
Expression system: synthetic construct [32630]
Length: 18 nucleotides
Theoretical weight: 5.59 KDa

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