› 6fhk

X-ray diffraction
1.66Å resolution

Structure of a modified protein containing a genetically encoded phosphoserine

Released:
DOI: 10.2210/pdb6fhk/pdb
PDB entry 6fhk coloured by chain, front view.
PDB entry 6fhk coloured by chain, side view.
PDB entry 6fhk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mitotic phosphorylation regulates Hsp72 spindle localization by uncoupling ATP binding from substrate release.
Mukherjee M, Sabir S, O'Regan L, Sampson J, Richards MW, Huguenin-Dezot N, Ault JR, Chin JW, Zhuravleva A, Fry AM, Bayliss R
Sci Signal 11 (2018)
PMID: 30108182

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock 70 kDa protein 1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 42.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV8 (Residues: 1-381; Coverage: 59%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand PO4 2 x PO4
Ligand MG 2 x MG
Ligand K 2 x K
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 51.625Å b: 110.876Å c: 63.483Å
α: 90° β: 90.41° γ: 90°
R-values:
R R work R free
0.164 0.162 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Hsp70 molecular chaperones: multifunctional allosteric holding and unfolding machines.
Clerico et al. (2019)
7 other articles

1 mention without citation

Mitotic phosphorylation regulates Hsp72 spindle localization by uncoupling ATP binding from substrate release.
Mukherjee et al. (2018)