› 6fez

X-ray diffraction
2.3Å resolution

Ryegrass mottle virus protease domain

Released:
DOI: 10.2210/pdb6fez/pdb
PDB entry 6fez coloured by chain, front view.
PDB entry 6fez coloured by chain, side view.
PDB entry 6fez coloured by chain, top view.
Source organism: Ryegrass mottle virus
Primary publication:
VPg Impact on Ryegrass Mottle Virus Serine-like 3C Protease Proteolysis and Structure.
Kalnins G, Ludviga R, Kalnciema I, Resevica G, Zeltina V, Bogans J, Tars K, Zeltins A, Balke I
Int J Mol Sci 24 (2023)
PMID: 36982419

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p2a2b Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 20.88 KDa
Source organism: Ryegrass mottle virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0MCW0 (Residues: 119-317; Coverage: 21%)
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 37.11Å b: 115.99Å c: 57.92Å
α: 90° β: 105.27° γ: 90°
R-values:
R R work R free
0.232 0.229 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Ryegrass mottle virus complete genome determination and development of infectious cDNA by combining two methods- 3' RACE and RNA-Seq.
Balke et al. (2023)

1 mention without citation

VPg Impact on Ryegrass Mottle Virus Serine-like 3C Protease Proteolysis and Structure.
Kalnins et al. (2023)