› 6fcq

X-ray diffraction
3.1Å resolution

The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A

Released:
DOI: 10.2210/pdb6fcq/pdb
PDB 6fcq coloured by chain and viewed from the front.
PDB 6fcq coloured by chain and viewed from the side.
PDB 6fcq coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa
Primary publication:
Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa.
Lee M, Batuecas MT, Tomoshige S, Domínguez-Gil T, Mahasenan KV, Dik DA, Hesek D, Millán C, Usón I, Lastochkin E, Hermoso JA, Mobashery S
Proc Natl Acad Sci U S A 115 4393-4398 (2018)
PMID: 29632171

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable soluble lytic transglycosylase Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZI6 (Residues: 30-642; Coverage: 99%)
Gene name: PA3020
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BUL 1 x BUL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P63
Unit cell:
a: 165.715Å b: 165.715Å c: 55.884Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.217
Expression system: Escherichia coli

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Citations

3 review citations

β-lactam Resistance in Pseudomonas aeruginosa: Current Status, Future Prospects.
Glen et al. (2021)
2 more