X-ray diffraction
2.5Å resolution

Crystal Structure of E. coli GyraseB 24kDa in complex with 6-[(ethylcarbamoyl)amino]-4-[(4-methoxyphenyl)amino]-N-(pyridin-3-yl)pyridine-3-carboxamide


Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.28 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
  • Canonical: P0AES7 (Residues: 1-220; Coverage: 27%)
Gene names: ECs4634, Z5190, gyrB
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 100.2Å b: 100.2Å c: 50.27Å
α: 90° β: 90° γ: 120°
R R work R free
0.209 0.207 0.238
Expression system: Escherichia coli