X-ray diffraction
1.9Å resolution

Crystal Structure of E. coli GyraseB 24kDa in complex with 4-(4-bromo-1H-pyrazol-1-yl)-6-[(ethylcarbamoyl)amino]-N-(pyridin-3-yl)pyridine-3-carboxamide


Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.65 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
  • Canonical: P0AES6 (Residues: 15-220; Coverage: 26%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3221
Unit cell:
a: 99.34Å b: 99.34Å c: 50.21Å
α: 90° β: 90° γ: 120°
R R work R free
0.204 0.203 0.219
Expression system: Escherichia coli