6f6w

Electron Microscopy
3.8Å resolution

Structure of Mycobacterium smegmatis RNA polymerase core

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.96 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QSL8 (Residues: 1-350; Coverage: 100%)
Gene names: MSMEG_1524, MSMEI_1488, rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1178 amino acids
Theoretical weight: 129.46 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: P60281 (Residues: 2-1169; Coverage: 100%)
Gene names: MSMEG_1367, MSMEI_1328, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1325 amino acids
Theoretical weight: 147.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QS66 (Residues: 2-1317; Coverage: 100%)
Gene names: MSMEG_1368, MSMEI_1329, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 107 amino acids
Theoretical weight: 11.51 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QWT1 (Residues: 2-107; Coverage: 99%)
Gene names: MSMEG_3053, MSMEI_2977, rpoZ
Sequence domains: RNA polymerase Rpb6

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4192
Expression system: Escherichia coli