› 6f5z

X-ray diffraction
1.35Å resolution

Complex between the Haloferax volcanii Trm112 methyltransferase activator and the Hvo_0019 putative methyltransferase

Released:
DOI: 10.2210/pdb6f5z/pdb
PDB entry 6f5z coloured by chain, front view.
PDB entry 6f5z coloured by chain, side view.
PDB entry 6f5z coloured by chain, top view.
Source organism: Haloferax volcanii DS2
Primary publication:
Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes.
van Tran N, Muller L, Ross RL, Lestini R, L��toquart J, Ulryck N, Limbach PA, de Cr��cy-Lagard V, Cianf��rani S, Graille M
Nucleic Acids Res. (2018)
PMID: 30010922

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112627 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyltransferase type 11 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26.3 KDa
Source organism: Haloferax volcanii DS2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: D4GYL4 (Residues: 1-226; Coverage: 100%)
Gene name: HVO_0019
Sequence domains: Methyltransferase domain
Methyltransferase activator Trm112 Chains: C, D
Molecule details ›
Chains: C, D
Length: 61 amino acids
Theoretical weight: 6.86 KDa
Source organism: Haloferax volcanii DS2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: D4GW82 (Residues: 1-61; Coverage: 100%)
Gene names: HVO_1131, trm112
Sequence domains: Trm112p-like protein

Ligands and Environments

2 bound ligands:
Ligand SAH 2 x SAH
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 80.61Å b: 82.29Å c: 88.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.207
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

10 review citations

Naturally occurring modified ribonucleosides.
McCown et al. (2020)
9 more

1 mention without citation

Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes.
van Tran et al. (2018)