› 6f5e

X-ray diffraction
2.7Å resolution

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_10_47 in complex JNK1a1 and JIP1 peptide

Released:
DOI: 10.2210/pdb6f5e/pdb
PDB entry 6f5e coloured by chain, front view.
PDB entry 6f5e coloured by chain, side view.
PDB entry 6f5e coloured by chain, top view.
Source organisms:
Entry authors: Wu Y, Mittl PR, Honegger A, Batyuk A, Plueckthun A

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155383 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DD_D12_10_47 Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 35.11 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Mitogen-activated protein kinase 8 Chain: B
Molecule details ›
Chain: B
Length: 373 amino acids
Theoretical weight: 43.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P45983 (Residues: 2-363; Coverage: 85%)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
C-Jun-amino-terminal kinase-interacting protein 1 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9WVI9 (Residues: 153-163; Coverage: 2%)
Gene names: Ib1, Jip1, Mapk8ip, Mapk8ip1, Prkm8ip

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P65
Unit cell:
a: 76.995Å b: 76.995Å c: 330.725Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.226
Expression systems:
  • Escherichia coli K-12
  • Not provided

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