› 6f5c

X-ray diffraction
1.55Å resolution

Structure of h. salinarum RosR (vng0258) grown from NaCl

Released:
DOI: 10.2210/pdb6f5c/pdb
PDB entry 6f5c coloured by chain, front view.
PDB entry 6f5c coloured by chain, side view.
PDB entry 6f5c coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
J Struct Biol 204 191-198 (2018)
PMID: 30110657

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator PadR N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Haloferax volcanii
UniProt:
  • Canonical: Q9HSF4 (Residues: 1-116; Coverage: 100%)
Gene names: VNG_0258H, rosR
Sequence domains: Transcriptional regulator PadR-like family

Ligands and Environments

2 bound ligands:
Ligand CL 25 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 40.824Å b: 71.35Å c: 41.879Å
α: 90° β: 110.749° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Haloferax volcanii

Quick links

Citations

1 citations in other articles

Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.
Kutnowski et al. (2019)