› 6f58

X-ray diffraction
2.25Å resolution

Crystal structure of human Brachyury (T) in complex with DNA

Released:
DOI: 10.2210/pdb6f58/pdb
PDB entry 6f58 coloured by chain, front view.
PDB entry 6f58 coloured by chain, side view.
PDB entry 6f58 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Newman JA, Gavard AE, Krojer T, Shrestha L, Burgess-Brown NA, von Delft F, Arrowsmith CH, Edwards A, Bountra C, Gileadi O

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119878 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
T-box transcription factor T Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15178 (Residues: 40-224; Coverage: 43%)
Gene names: T, TBXT
Sequence domains: T-box
Structure domains: Transcription factor, T-box
DNA (5'-D(*AP*AP*TP*TP*TP*CP*AP*CP*AP*CP*CP*TP*AP*GP*GP*TP*GP*TP*GP*AP*AP*AP*TP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 74.978Å b: 37.109Å c: 110.796Å
α: 90° β: 103.99° γ: 90°
R-values:
R R work R free
0.244 0.242 0.288
Expression systems:
  • Escherichia coli
  • Not provided

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