› 6f3t

X-ray diffraction
2.5Å resolution

Crystal structure of the human TAF5-TAF6-TAF9 complex

Released:
DOI: 10.2210/pdb6f3t/pdb
PDB entry 6f3t coloured by chain, front view.
PDB entry 6f3t coloured by chain, side view.
PDB entry 6f3t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly.
Antonova SV, Haffke M, Corradini E, Mikuciunas M, Low TY, Signor L, van Es RM, Gupta K, Scheer E, Vos HR, Tora L, Heck AJR, Timmers HTM, Berger I
Nat Struct Mol Biol 25 1119-1127 (2018)
PMID: 30510221

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156065 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcription initiation factor TFIID subunit 5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 608 amino acids
Theoretical weight: 68.95 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q15542 (Residues: 194-800; Coverage: 76%)
Gene names: TAF2D, TAF5
Sequence domains:
Transcription initiation factor TFIID subunit 6 Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 94 amino acids
Theoretical weight: 10.89 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P49848 (Residues: 5-92; Coverage: 13%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains: TATA box binding protein associated factor (TAF)
Transcription initiation factor TFIID subunit 9 Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 116 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q16594 (Residues: 5-120; Coverage: 44%)
Gene names: TAF2G, TAF9, TAFII31
Sequence domains: Transcription initiation factor IID, 31kD subunit

Ligands and Environments

1 bound ligand:
Ligand CL 7 x CL
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I23
Unit cell:
a: 339.05Å b: 339.05Å c: 339.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.22
Expression system: unidentified baculovirus

Quick links

Citations

5 review citations

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
4 more

4 mentions without citation

Structure of the transcription coactivator SAGA.
Wang et al. (2020)
3 more