› 6f0t

X-ray diffraction
1.74Å resolution

Crystal structure of Pizza6-SFW

Released:
DOI: 10.2210/pdb6f0t/pdb
PDB 6f0t coloured by chain and viewed from the front.
PDB 6f0t coloured by chain and viewed from the side.
PDB 6f0t coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies.
Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A
Biochem. Biophys. Res. Commun. 497 1038-1042 (2018)
PMID: 29481797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pizza6-SFW Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 256 amino acids
Theoretical weight: 25.84 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 32.529Å b: 69.454Å c: 78.71Å
α: 65.36° β: 89.8° γ: 77.16°
R-values:
R R work R free
0.194 0.192 0.233
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Influence of circular permutations on the structure and stability of a six-fold circular symmetric designer protein.
Mylemans et al. (2020)
1 more