› 6f0c

X-ray diffraction
1.7Å resolution

Cytochrome P450 TxtC employs substrate conformational switching for sequential aliphatic and aromatic thaxtomin hydroxylation

Released:
DOI: 10.2210/pdb6f0c/pdb
PDB entry 6f0c coloured by chain, front view.
PDB entry 6f0c coloured by chain, side view.
PDB entry 6f0c coloured by chain, top view.
Source organism: Streptomyces acidiscabies 84-104
Primary publication:
Binding of Distinct Substrate Conformations Enables Hydroxylation of Remote Sites in Thaxtomin D by Cytochrome P450 TxtC.
Alkhalaf LM, Barry SM, Rea D, Gallo A, Griffiths D, Lewandowski JR, Fulop V, Challis GL
J Am Chem Soc 141 216-222 (2019)
PMID: 30516965

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 43.47 KDa
Source organism: Streptomyces acidiscabies 84-104
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VS75 (Residues: 1-395; Coverage: 100%)
Gene name: txtC
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand C8B 1 x C8B
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 117.4Å b: 84.5Å c: 84.4Å
α: 90° β: 133.16° γ: 90°
R-values:
R R work R free
0.163 0.162 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Enzymatic Late-Stage Modifications: Better Late Than Never.
Romero et al. (2021)
10 other articles