› 6ez1

X-ray diffraction
1.75Å resolution

Structure of h. salinarum RosR (vng0258) grown from NaBr

Released:
DOI: 10.2210/pdb6ez1/pdb
PDB 6ez1 coloured by chain and viewed from the front.
PDB 6ez1 coloured by chain and viewed from the side.
PDB 6ez1 coloured by chain and viewed from the top.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
The 3-D structure of VNG0258H/RosR - a haloarchaeal DNA-binding protein in its ionic shell.
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
J. Struct. Biol. (2018)
PMID: 30110657

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator PadR N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Haloferax volcanii
UniProt:
  • Canonical: Q9HSF4 (Residues: 1-116; Coverage: 100%)
Gene names: VNG_0258H, rosR
Sequence domains: Transcriptional regulator PadR-like family

Ligands and Environments

2 bound ligands:
Ligand BR 33 x BR
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 40.884Å b: 71.139Å c: 41.719Å
α: 90° β: 110.334° γ: 90°
R-values:
R R work R free
0.172 0.17 0.209
Expression system: Haloferax volcanii

Quick links

Citations

1 citations in other articles

Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.
Kutnowski et al. (2019)