› 6eyt

X-ray diffraction
2.21Å resolution

Crystal structure of the Salmonella effector SseK3 in complex with UDP-GlcNAc and Manganese

Released:
DOI: 10.2210/pdb6eyt/pdb
PDB entry 6eyt coloured by chain, front view.
PDB entry 6eyt coloured by chain, side view.
PDB entry 6eyt coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3.
Esposito D, Günster RA, Martino L, El Omari K, Wagner A, Thurston TLM, Rittinger K
J Biol Chem 293 5064-5078 (2018)
PMID: 29449376

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type III secretion system effector arginine glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.82 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A0H3NMP8 (Residues: 14-335; Coverage: 96%)
Gene names: G1W50_07705, G1W53_11560, G1W54_11945, G1W56_12450, G1W63_12255, G1W64_12270, G1W86_10855, G1W87_22055, G1W89_11745, G1X03_12135, G1X09_12595, G1X10_12510, G1X13_12590, G1X15_07355, G1X17_12600, G1X23_12495, G1X32_11650, G1X38_11955, G1X47_10960, G1X49_12150, G1X51_12595, G1X66_11955, G1X68_11040, G1X69_12865, G1X71_12600, G1X76_11625, G1X80_11625, G1X84_19410, G1X85_22430, G1X86_11055, G1X94_11480, G1X96_11060, G1X99_12285, G1Y02_11655

Ligands and Environments

4 bound ligands:
Ligand IOD 4 x IOD
Ligand MN 2 x MN
Ligand UDP 2 x UDP
Ligand NDG 2 x NDG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 73.97Å b: 85.94Å c: 96.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.241
Expression system: Escherichia coli K-12

Quick links

Citations

5 review citations

Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.
Araujo-Garrido et al. (2020)
4 more

2 mentions without citation

Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3.
Esposito et al. (2018)
1 more