› 6ewn

X-ray diffraction
2.29Å resolution

HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation

Released:
DOI: 10.2210/pdb6ewn/pdb
PDB 6ewn coloured by chain and viewed from the front.
PDB 6ewn coloured by chain and viewed from the side.
PDB 6ewn coloured by chain and viewed from the top.
Source organism: Thermostichus vulcanus
Primary publication:
Biophysical and structural characterization of the small heat shock protein HspA from Thermosynechococcus vulcanus in 2 M urea.
Ghosh S, Salama F, Dines M, Lahav A, Adir N
Biochim Biophys Acta Proteins Proteom 1867 442-452 (2019)
PMID: 30711645

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SHSP domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.13 KDa
Source organism: Thermostichus vulcanus
Expression system: Escherichia coli
UniProt:
  • Canonical: O82825 (Residues: 37-135; Coverage: 68%)
Gene name: hspA
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand URE 44 x URE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I41
Unit cell:
a: 88.65Å b: 88.65Å c: 114.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.28 0.342
Expression system: Escherichia coli

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