› 6evg

X-ray diffraction
1.1Å resolution

Structural and Functional Characterisation of a Bacterial Laccase-like Multi-copper Oxidase CueO from Lignin-Degrading Bacterium Ochrobactrum sp. with Oxidase Activity towards Lignin Model Compounds and Lignosulfonate

Released:
DOI: 10.2210/pdb6evg/pdb
PDB entry 6evg coloured by chain, front view.
PDB entry 6evg coloured by chain, side view.
PDB entry 6evg coloured by chain, top view.
Source organism: Ochrobactrum
Primary publication:
Structural and functional characterisation of multi-copper oxidase CueO from lignin-degrading bacterium Ochrobactrum sp. reveal its activity towards lignin model compounds and lignosulfonate.
Granja-Travez RS, Wilkinson RC, Persinoti GF, Squina FM, Fülöp V, Bugg TDH
FEBS J 285 1684-1700 (2018)
PMID: 29575798

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 55.14 KDa
Source organism: Ochrobactrum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6X5N0 (Residues: 30-33, 34-397, 398-533; Coverage: 95%)
Gene name: Oant_3828
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 47.33Å b: 48.31Å c: 88.85Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.126 0.125 0.149
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives.
Lee et al. (2019)
6 more

2 mentions without citation

Structural and functional characterisation of multi-copper oxidase CueO from lignin-degrading bacterium Ochrobactrum sp. reveal its activity towards lignin model compounds and lignosulfonate.
Granja-Travez et al. (2018)
1 more