› 6eux

X-ray diffraction
2.05Å resolution

Structure of the midlink and cap-binding domains of influenza B polymerase PB2 subunit with a bound azaindazole cap-binding inhibitor

Released:
DOI: 10.2210/pdb6eux/pdb
PDB 6eux coloured by chain and viewed from the front.
PDB 6eux coloured by chain and viewed from the side.
PDB 6eux coloured by chain and viewed from the top.
Source organism: Influenza B virus (B/Memphis/13/2003)
Primary publication:
Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Pflug A, Gaudon S, Resa-Infante P, Lethier M, Reich S, Schulze WM, Cusack S
OpenAccess logo Nucleic Acids Res 46 956-971 (2018)
PMID: 29202182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.01 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5V8X3 (Residues: 249-540; Coverage: 38%)
Gene name: PB2

Ligands and Environments

1 bound ligand:
Ligand BYB 1 x BYB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P3121
Unit cell:
a: 73.2Å b: 73.2Å c: 103.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structural insights into RNA polymerases of negative-sense RNA viruses.
Te Velthuis et al. (2021)
1 more