› 6eup

X-ray diffraction
2.65Å resolution

Crystal structure of Neisseria meningitidis NadA variant 3 double mutant A33I-I38L

Released:
DOI: 10.2210/pdb6eup/pdb
PDB entry 6eup coloured by chain, front view.
PDB entry 6eup coloured by chain, side view.
PDB entry 6eup coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
NadA3 Structures Reveal Undecad Coiled Coils and LOX1 Binding Regions Competed by Meningococcus B Vaccine-Elicited Human Antibodies.
Liguori A, Dello Iacono L, Maruggi G, Benucci B, Merola M, Lo Surdo P, López-Sagaseta J, Pizza M, Malito E, Bottomley MJ
mBio 9 (2018)
PMID: 30327444

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neisseria adhesin A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 156 amino acids
Theoretical weight: 16.76 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DV44 (Residues: 24-170; Coverage: 39%)
Gene names: nadA, nadA3

Ligands and Environments

5 bound ligands:
Ligand CL 1 x CL
Ligand EDO 18 x EDO
Ligand MPD 5 x MPD
Ligand P6G 2 x P6G
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2
Unit cell:
a: 68.919Å b: 39.75Å c: 255.63Å
α: 90° β: 95.13° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli

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Citations

4 review citations

Vaccines against Meningococcal Diseases.
Pizza et al. (2020)
3 more

2 mentions without citation

NadA3 Structures Reveal Undecad Coiled Coils and LOX1 Binding Regions Competed by Meningococcus B Vaccine-Elicited Human Antibodies.
Liguori et al. (2018)
1 more