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Structural basis of RNA polymerase III transcription initiation.

Abascal-Palacios G, Ramsay EP, Beuron F, Morris E, Vannini A
Nature 553 301-306 (2018)
Related entries: 6eu0, 6eu1, 6eu2

Cited: 78 times
EuropePMC logo PMID: 29345637

Abstract

RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene transcription by Pol III requires the activity of the transcription factor TFIIIB to form a transcriptionally active Pol III preinitiation complex (PIC). Here we present electron microscopy reconstructions of Pol III PICs at 3.4-4.0 Å and a reconstruction of unbound apo-Pol III at 3.1 Å. TFIIIB fully encircles the DNA and restructures Pol III. In particular, binding of the TFIIIB subunit Bdp1 rearranges the Pol III-specific subunits C37 and C34, thereby promoting DNA opening. The unwound DNA directly contacts both sides of the Pol III cleft. Topologically, the Pol III PIC resembles the Pol II PIC, whereas the Pol I PIC is more divergent. The structures presented unravel the molecular mechanisms underlying the first steps of Pol III transcription and also the general conserved mechanisms of gene transcription initiation.

Articles - 6eu3 mentioned but not cited (2)

  1. Structural basis for RNA polymerase III transcription repression by Maf1. Vorländer MK, Baudin F, Moir RD, Wetzel R, Hagen WJH, Willis IM, Müller CW. Nat Struct Mol Biol 27 229-232 (2020)
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Reviews citing this publication (19)

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Articles citing this publication (57)

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