6es1

X-ray diffraction
2Å resolution

Crystal structure of the binding domain from botulinum neurotoxin A2 bound to extracellular domain of human receptor SV2C

Released:

Function and Biology Details

Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin A2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 445 amino acids
Theoretical weight: 51.64 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q45894 (Residues: 874-1296; Coverage: 33%)
Gene names: CLM_0897, atx, bna, bonT, botA
Sequence domains:
Structure domains:
Synaptic vesicle glycoprotein 2C Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 13.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q496J9 (Residues: 474-567; Coverage: 13%)
Gene names: KIAA1054, SV2C
Structure domains: E3 ubiquitin-protein ligase SopA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 100.714Å b: 122.108Å c: 47.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.23
Expression system: Escherichia coli BL21(DE3)