› 6epb

X-ray diffraction
1.75Å resolution

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endochitinase 42 Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 46.07 KDa
Source organism: Trichoderma harzianum
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P48827 (Residues: 1-423; Coverage: 100%)
Gene name: chit42
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 7 x ZN
Ligand EDO 20 x EDO
Ligand ACT 4 x ACT
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P41212
Unit cell:
a: 68.353Å b: 68.353Å c: 178.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.219
Expression system: Komagataella phaffii GS115

Quick links

Citations

7 review citations

Seafood Waste as Attractive Source of Chitin and Chitosan Production and Their Applications.
Santos et al. (2020)
6 more

4 mentions without citation

Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity.
Kidibule et al. (2018)
3 more