6eou

X-ray diffraction
1.75Å resolution

O-GlcNAc transferase TPR domain with the intellectual disability associated mutation L254F

Released:

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15294 (Residues: 26-410; Coverage: 37%)
Gene name: OGT
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2221
Unit cell:
a: 45Å b: 203.162Å c: 116.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.227
Expression system: Escherichia coli BL21(DE3)