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X-ray diffraction
1.17Å resolution

Tryptophan Repressor TrpR from E.coli variant T44L T81M N87G S88Y with Indole-3-acetic acid as ligand

Released:
DOI: 10.2210/pdb6enn/pdb
PDB 6enn coloured by chain and viewed from the front.
PDB 6enn coloured by chain and viewed from the side.
PDB 6enn coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
A biosensor for the direct visualization of auxin
Herud-Sikimic O, Stiel AC, Kolb M, Shanmugaratnam S, Berendzen KW, Feldhaus C, Hocker B, Juergens G
Nature (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trp operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A881 (Residues: 1-108; Coverage: 100%)
Gene names: JW4356, b4393, rtrY, trpR
Sequence domains: Trp repressor protein
Structure domains: TrpR-like

Ligands and Environments

1 bound ligand:
Ligand IAC 2 x IAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 54.577Å b: 63.185Å c: 64.904Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.18
Expression system: Escherichia coli BL21

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